Ctgf sigma
WebConnective tissue growth factor (CTGF) is a signaling molecule with several roles in tissue repair and regeneration including promoting cell adhesion, cell migration, cell proliferation and angiogenesis. ... (MTT) used in this work were purchased from Sigma-Aldrich. Poly-L-lactic acid (PLLA, Mn 125-120) was obtained from Akina, US. Phosphate ... WebConnective tissue growth factor (CTGF) is a member of the CTGF, cyr 61/cef 10, nov (CCN) protein family, which participates in a wide variety of biological processes such as embryonic development and tissue repair. 1,2 CTGF itself is a potent and ubiquitously expressed growth factor for fibroblasts, chondrocytes and vascular endothelial cells. 2 …
Ctgf sigma
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WebAug 5, 2024 · Connective tissue growth factor (CTGF) has been recently acknowledged as an ideal biomarker in the early disease course, participating in the pathogenesis of pannus formation in rheumatoid arthritis (RA). ... (Sigma-Aldrich, Saint Louis, USA) in 1 × TAE buffer and visualized with GelRed (Sigma-Aldrich). The lanes were quantified and … WebMay 6, 2024 · This study aimed to investigate the mechanisms by which the expression of connective tissue growth factor (CTGF), a downstream mediator of TGF-β, is controlled by miRNAs post-transcriptionally after exposure to ionizing radiation. ... SB431542, an inhibitor of TGF-β type I receptor, and a siRNA-targeting mouse CTGF (SIGMA, St. …
WebNov 2, 2024 · Connective-tissue growth factor (CTGF), also known variously as CCN2 (Cyr61 [cysteine-rich protein 61] and NOV [nephroblastoma overexpressed gene], ... (Sigma-Aldrich), sodium deoxycholate, and NaCl and stored in PBS supplemented with penicillin–streptomycin. WebApr 13, 2024 · The identification of tissue-specific promoters for gene therapeutic constructs is one of the aims of complex tumor therapy. The genes encoding the fibroblast activation protein (FAP) and the connective tissue growth factor (CTGF) can function in tumor-associated stromal cells but are practically inactive in normal adult cells. Accordingly, the …
WebSep 17, 2024 · Elevated expression of markers of gingival fibrosis (particularly CTGF), markers of epithelial–mesenchymal transition (EMT), periostin, and enzymes of the lysyl oxidase family have been documented in DIGO. ... (DMEM) (Sigma–Aldrich, St. Louis, MO, USA) supplemented with 10% foetal bovine serum (FBS; Gibco, Grand Island, NY, … WebNov 19, 2024 · Two alternations to the method were introduced—the incubation with the primary antibody was performed for 60 minutes at room temperature instead of overnight incubation at 4 °C, and in the case of CTGF, antigenic sites were exposed by incubation with Proteinase K (Sigma-Aldrich, Saint Louis, MO, USA) for 10 minutes at room temperature.
WebJan 30, 2024 · Background TGFβ1 is a major profibrotic mediator in chronic kidney disease (CKD). Its direct inhibition, however, is limited by adverse effects. Inhibition of activins, also members of the TGFβ superfamily, blocks TGFβ1 profibrotic effects, but the mechanism underlying this and the specific activin(s) involved are unknown. Methods Cells were …
WebFeb 1, 2024 · Doc-00LAJM；本文是的论文参考范文或相关资料文档。正文共3,125字，word格式文档。内容摘要：【摘要】 目的：研究转化生长因子（TGF-β2）和结缔组织生长因子(CTGF)在滤过泡瘢痕化中的作用。 方法：用不同浓度的TGF-β2刺激HTF，然后用RT-PCR和免疫细胞化学法分别检测CTGF和Fn的表达，用MTT法检测细胞的 ... citizenship in the community bsa merit badgeWebCCN (for CYR61/ CTGF /NOV) proteins were identified originally from diverse cellular contexts and studied for their growth-promoting activities. It is now known that there are … citizenship in the community merit badge pptWebMar 21, 2024 · GeneCards Summary for CCN2 Gene. CCN2 (Cellular Communication Network Factor 2) is a Protein Coding gene. Diseases associated with CCN2 include Renal Fibrosis and Systemic Scleroderma . Among its related pathways are Apoptotic Pathways in Synovial Fibroblasts and GPCR Pathway . An important paralog of this gene is CCN3. citizenship in the community boy scoutsWebJan 27, 2024 · A cysteine-rich matricellular protein connective tissue growth factor (CTGF, also known as CCN2), a member of the CCN (Cry61, CTGF, and Nov) family, is a molecule regulating a number of biological ... citizenship in the community definitionWebApr 7, 2024 · Tissues were frozen in isopentane (Sigma‐Aldrich, USA) and then embedded in optimal cutting temperature compound (OCT). The embedded tissue blocks were cryosectioned in a cryostat to generate appropriately sized sections for Visium Spatial slides while keeping the samples frozen. ... (MC; n = 277) marked by CTGF, and podocytes … dickhof wezepWebCTGF (connective tissue growth factor) regulates cell communication with the extracellular matrix also inducing collagen deposition, stimulation and differentiation of … citizenship in the community bsa worksheetWebConnective tissue growth factor (CTGF), also termed fisp12, β IG-M2, and IGF-BP8, is a secreted cysteine-rich growth factor that belongs to the CCN (cyr61, CTGF, nov) family. It was first isolated in 1991 from serum-stimulated NIH3T3 cells and named fisp12 (fibroblast-inducible secreted protein-12). The term ‘connective tissue growth factor ... citizenship in the community merit badge 2022