WebFeb 24, 2024 · CTLL-2 proliferation assay. In order to starve the CTLL-2 cells from IL-2, the cells were washed twice with prewarmed HBSS (Gibco, #14175095) and then grown in the absence of IL-2 for 24 h in RPMI ... WebApr 5, 2024 · measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 0.0300-0.250 ng/mL. Two independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of Animal-FreeTM Recombinant Human IL-2. %LRDFWLYLW\
Mouse IL-15/IL-15R Complex Recombinant Protein, …
WebThe biological activity of the recovered soluble rhIL-2 protein was determined in a proliferation assay using CTLL-2 cells. Human IL-2 purchased from Sigma-Aldrich Co. … WebFor example, Recombinant Human IL-2 can activate proliferation of IL-2 dependent cell lines, CTLL-2 and HT-2. The same lot of recombinant protein provides different ED 50 values depending on the cell line used for stimulation. In the CTLL-2 stimulation assay, the ED 50 is 0.26 ng/ml corresponding to a specific activity of 3.73 x10 6 units/mg. flow speed formula
CTLL-2 Cell Line - an overview ScienceDirect Topics
WebThe bioactivity of the superagonist was characterized by CTLL-2 cells proliferation assay in vitro, showing its capability of stimulating the expansion of memory CD8 + T cells (cluster of differentiation) in mouse spleen. WebWhen maintained in continuous culture, however, the overall sensitivity of this cell line is highly variable. To overcome this problem, large batches of CTLL-2 were grown, frozen and tested for their ability to detect low levels of IL-2 (0.01 IU) in dose response assays. Batches meeting this criterion were stored in liquid nitrogen until required. WebCTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines. Culture Medium RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human … green color psychology impact