Tīmeklissoftware version 6.0 and later with BD LSR II flow cytometers. Workflow Overview. The following figure shows the steps for daily workflow using BD FACSDiva software. …
BD LSR-II Instruments University of Groningen
TīmeklisHarvest, cleaning the cells (single cell suspension) and adjust lockup number to a focus of 1-5x106 cells/ml in ice cold FACS Store (PBS, 0.5-1% BSA or 5-10% http://highchurchwesleyan.com/live-cell-antibody-staining-protocol tesco warsaw
Chimerism and phenotypic analysis of intraepithelial and lamina …
TīmeklisLSR II has the capability for adjusting the flow rate for various applications. The three preset flow rates are: LO = 12 µl/min, MED = 35 μl/min, HI = 60 μl/min. LSR II is … TīmeklisTo help with pinpointing exact data, the analyzer has the capacity to zoom in, allowing you easily visualize spectra intersection points with laser lines, filter sets and other spectra. To zoom in, double click your mouse over the area that you wish to zoom, or roll the mouse wheel forward. To zoom out, click on the zoom out button at the top ... TīmeklisHarvest, launder the single (single cell suspension) and adjust cell number to a concentration of 1-5x106 cells/ml on ice cold FACS Buffer (PBS, 0.5-1% BSA or 5-10% tesco washing up bowl and drainer