NettetDownload scientific diagram Immunofluorescence analysis of hepatic markers. Immunofluorescence micrographs (merged) showing positive expression of hepatocyte specific markers, AFP (Alexa Fluor ... Nettet20. jul. 2024 · Several cell surface markers, such as CD13 and DLK1, were found to label and isolate hepatoblasts/hepatocytes from the mouse fetal liver. 45,46,47 Based on …
Hepatocyte differentiation of mesenchymal stem cells from human …
NettetWestern blotting was used to detect specific exosomal surface markers, which were encapsulated in exosomes ... Membranes were then incubated with horseradish peroxidase-conjugated anti-rabbit IgG and anti-mouse IgG antibodies (1:2,000, Thermo Fisher Scientific ... Western blot detection of hepatocyte autophagy markers LC3B … NettetYou can use CD13 and DLK1 as cell surface markers for hepatocytes in mouse fetal liver. Please refer to the research article attached under the heading, Identification of novel … i 75 through kentucky map
Liver progenitor cell-driven liver regeneration Experimental
NettetPrimary hepatocyte cultures better reflect the properties of the liver in vivo than do cell lines derived from the liver. Here we describe a method for the isolation and culture of … Nettet16. jul. 2024 · A substantial amount of research is being conducted on zonation markers to identify hepatic injuries and disorders based on the structural and functional zonation of the liver. In contrast to metabolic zonation, hepatocyte ploidy reflects the capability of liver regenerative turnover. Nonetheless, many knowledge gaps remain in the … NettetPrepare single-cell suspensions from mouse thymus or spleen in PBS with 5% FBS (no azide). Wash once with PBS with 5% FBS. Keep the cells at 4°C before stimulation. Treat the cells (example: use 100 μl of cell suspension) with the selected activator and/or inhibitor for the selected period of time. molly yeh potato soup